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Exosomal Marker CD63 Antibody Set Kit

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Exosomes are small membrane-bound vesicles that in recent years have emerged as important molecules for inter-cellular communication. Exosomes are produced during both normal and patho/physiological conditions, and cancer cells have been shown to secrete exosomes in greater amounts than normal cells. Anti-CD63 recognizes an extracellular fragment of CD63, a 56-kilodalton (kDa), type III lysosomal glycoprotein, belonging to the tetraspanin family. CD63 is expressed by granulocytes, platelets, T-cells, monocytes/macrophages, endothelial cells. CD63 protein is also expressed in exosome membrane. CD63 protein is a canonical exosome marker and currently used to characterize exosome populations from a variety of body fluids. These glycosylations can affect the size at which the marker proteins appear on a Western blot and bands may appear at several different sizes.
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The Exosomal Marker (CD63) Antibody Set Kit provides an economical means to evaluate the presence of exosomal markers. The kit includes enough primary antibody to perform at least 10 western blot experiments for each target. The secondary antibody that is included in this kit has been optimized to enhance the signal-to-noise ratio. We do not recommend using other secondary antibodies with the primary antibodies at this time.


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